Drug discovery enabling technology

Molecular dynamics studies of proteins of different sizes including membrane-bound proteins, protein-ligand complexes, and drug delivery systems;

QM-level calculations of crystallographic structures, small and large molecules;

Molecular docking;

High-throughput virtual screening;

Molecular target identification using metabolomics and other omic databases;

Hit-to-lead, lead optimization;

Fragment-based lead optimization and ligand-based molecular design;

Targeted libraries creation;

ADMET predictions

QSAR/AutoQSAR. Building and working with ANNs (Canvas, TensorFlow, other);

Drug repurposing;

Molecular design de novo, including the big molecules (peptides, aptamers etc);

Prediction of possible effects, targets and activity mechanisms of various compounds;

De novo molecular design in multiple modalities (small molecular, peptides, aptamers, etc.);

Predictive pharmacology including polypharmacology effects;

Ligand design and optimization for particular target;

Python\BioPython\Scala\C++\Fortran etc programming for biology and pharmacology.

Our advanced and flexible medicinal chemistry-focused proprietary technology platform enables us to utilize effectively our synthetic, medicinal, and computational chemistry resources to successfully design and produce a wide range of diverse, targeted, focused chemical libraries or follow-up and hit-to-lead chemical libraries. We can generate chemical libraries to all our partners’ varied requirements of scale, purity, and formats.

Research, development, and production of chemicals can be produced on a non-exclusive, semi-exclusive or exclusive basis for partners. These molecules leverage the advanced, chemistry-focused, proprietary technology platform that was developed by Advanced Molecular Technologies for the generation of novel biologically active compounds.

Synthesis of individual analogs and parallel synthesis.

High-throughput screening and evaluation of biological effects and toxicological properties on animal models

Scale-up capabilities for preclinical testing.

Synthetic method development:

  • Microwave assisted technics
  • Solution chemistry reactors
  • High-pressure hydrogenation
  • Inert atmosphere Schlenk-line technics
  • Preparative High and Medium pressure chromatography
  • High-vacuum distillation
  • Liophilization

Preclinical development on the base of the Institute of Translational Medicine and Biotechnology:

Available resources of Institute of Translational Medicine and Biotechnology afford comprehensive support in carrying out drugs formulation and targeted drug delivery systems development:

Development of drug-loaded targeted delivery systems based on natural or synthetic polymers and surfactants

Development of anticancer nanoscale targeted drug delivery systems providing transport across the blood-brain barrier

Development of antibiotics- squalene- or hyaluronic acid-loaded polymer-based nanoparticles;

Surface vectorization of nanoscale targeted drug delivery systems;

Study of in vitro drug distribution;

Determination of intravenous administrated drugs pharmacokinetic parameters.

Development of nanosomal drug formulations by nanoprecipitation, a single emulsion or a double emulsion methods;

Investigation of nanosomal drug formulation release kinetics using different methods (UV, CE, HPLC and et al);

Development of nanoparticles surface vectorization with or without linker use;

Research of fluorescent tags introduction into nanosomal formulations;

New drug formulation in vitro/in vivo assay;

Investigation of nanosomal anticancer (or antibiotics) formulations composition influence on biodistribution;

Advanced equipment:

  • Microfluidics, Great Britain (Dolomite provides microfluidics based solutions for a range of applications including drug encapsulation, droplet manufacture and particle generation);
  • Nanotrac Flex, Microtrac Inc., USA (Measures particles ranging in size from 0.8 to 6,500 nanometers and size distribution);
  • Stabino, Microtrac Inc., USA (Zeta potential instrument for analysing and optimising the stability of colloids and dispersions, based on measurement of streaming potential. Employing a unique oscillating piston design, the system incorporates rapid pH and polyelectrolyte titrations for investigation of stable pH regions, Iso-Electric Point (IEP), charge density and surface functionalization);
  • EmulsiFlex-C3, Avestin, Germany (Lab scale, electric motor driven, high pressure homogeniser that rapidly reduces particles and droplets from micron to nanometer sizes.);
  • Alpha 2-4 LSCPlus, Martin Christ GmbH, Germany (Compact tabletop unit for effective lyophilisation of specimens with innovative Wireless Shelf Technology. The simple and intuitive LSCplus controller supports manual and automatic process control for reproducible results).

All types of preclinical studies on laboratory animals (rodent: mice, rats, hamsters, guinea pigs. Non-rodent: rabbits, dogs, mini-pigs, macabi crab-eaters).

All types of administration of drugs (intravenous, subcutaneous, intradermal, intraperitoneal, peroral, rectal, intrabulbar, intracardiac et al.)

Methodology:

Pharmacokinetics

Pharmacodinamics

Acute toxicity

Chronic toxicity

Reproductive toxicity
Study of reproductive (generative) function of animals
Study of embryotoxic and fetotoxic action
Study of antinatal action

Mutagenicity
Mammalian Bone Marrow Chromosome Aberration Test
Rodent dominant lethal assay

Allergenicity
Active cutaneous anaphylaxis
General anaphylaxis
Conjunctival test
Method of cutaneous applications
Delayed-Type Hypersensitivity

Immunotoxicity
Determination of the number of antibody-forming cells
Assessment of phagocytic activity of peritoneal macrophages
DTH

Cumulation

Cross-reactivity

Behavioral tests (open field, Plus Maze, Morris test, dark-light camera, Beam walking, etc.)

Attestation of bank cell lines (in vivo part)

Experimental modeling:
Streptozotocin-induced and alloxan-induced diabetes
Antigen-induced arthritis
Compliment-induced autoimmune disease
Autoimmune encephalomyelitis
Orthotopic tumor models
Xenografts

PHASES:

  • Phase I - IV
  • Bioequivalence

MEDICAL AREAS:

  • Cardiology
  • Neurology
  • Rheumatology
  • Oncology
  • Pulmonology
  • Nephrology and other

Documentation package preparation:

  • Registration dossier
  • Protocol
  • Brochure
  • Informed consent form
  • Final report
  • Individual consultations

Site-proprietary pool of healthy volunteers (more than 1500 people) with special groups (healthy postmenopausal women, smokers and etc.)
Site-proprietary of patients (rheumatology, cardiology, endocrinology, pulmonology, etc.)

Multifunctional beds
Shower rooms
Alarm buttons
Synchronized clocks
24/7 ССTV cameras
Free Wi-fi
Recreation room
Two treatment rooms with modern equipment for parenteral manipulations, emergency procedures and first aid kit
Modern medical supplies